![]() RNA purified by other methods can be further purifiedĮnzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desaltingĮnzymes and excess NTPs are removed to yield highly pure synthesized RNAįractionation of RNA into small and large RNA poolsįigure 2: The Monarch RNA Cleanup Kit (500 µg) is suitable for cleaning up large quantities (>250 µg) of RNA from in vitro transcription reactionsĪ. RNA Cleanup and Concentration (including from the TRIzol aqueous phase) RNA Labeling, RNAi, Microinjections, RT-PCR, RNA library prep for NGS, transfection ≥ 25 nt ( ≥ 15 nt with modified protocol)ġ0–15 minutes of spin and incubation time Specifications and Applications: SPECIFICATIONSĬleanup of RNA from large-scale in vitro transcription reactions Columns and buffers are also available separately for convenience.įigure 1: Monarch RNA Cleanup Kit workflow Monarch RNA Cleanup Kits are also available for 10 µg ( NEB #T2030) and 50 µg ( NEB #T2040) binding capacities. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nt).ĭesigned with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 μl. ![]() The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The Monarch RNA Cleanup Kit (500 µg) reliably purifies up to 500 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. ![]()
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